Day two of the League of Legends Mid-Season Invitational 2021 has concluded, and North American fans will still have to wait for their regional representative's first victory on the tourney.The results of the first day were nothing out of the blue, as all group favorites comfortably moved a step ahead towards the rumble stage. Meanwhile, the second day of MSI 2021 saw the tournament's first major upset, as Cloud9 failed to win their second successive match, this time against Japanese representatives, DetonatioN FocusMe.The #MSI2021 Standings after Day 2! pic.twitter.com/4SKD4TTAFA— LoL Esports (@lolesports) May 7, 2021The European, Chinese, and Korean Spring Split champions maintained their undefeated winning streak. DWG Kia and MAD Lions extended their score to 2-0, while Royal Never Give Up won their third successive game in the tournament..@PSG_Talon win against @paiNGamingBR! #MSI2021 pic.twitter.com/yzuD9XcHoJ— LoL Esports (@lolesports) May 7, 2021Even though the North American champions failed to live up to expectations, some of the minor regional representatives, like PSG Talon and DetonatioN FocusMe, tasted their first victories in the League of Legends MSI 2021. After another six action-packed games, here's how things stand at the end of the second day of play.Results and standings of League of Legends MSI 2021 after Groups Day 2The results of Groups Day 1:Pentanet.GG 0-1 Royal Never Give UpUnicorns of Love 0-1 Royal Never Give UpGillette Infinity 0-1 DWG KIADetonatioN FocusMe 1-0 Cloud9İstanbul Wildcats 0-1 MAD LionsPaiN Gaming 0-1 PSG TalonGroup-wise standings after GD 2:Group ARoyal Never Give Up (3-0)Unicorns of Love (1-1)Pentanet.GG (0-3)Group BMAD Lions (2-0)PaiN Gaming (1-1)PSG Talon (1-1)İstanbul Wildcats01 (0-2)Group CDWG KIA (2-0)DetonatioN FocusMe (1-1)Gillette Infinity (1-1)Cloud9 (0-2)MSI 2021 day 2 saw the tournament's first "underdog disappointment" as DetonatioN FocusMe snatched away victory points from Cloud9Cloud9's defeat to DFM came as a shock to fans, as the League of Legends Japan League's spring champions thrashed the North American powerhouse within just 30 minutes of play. Robert "Blaber" Huang and co. found themselves standing at a gold deficit of 8800 when their nexus was in the hands of DetonatioN FocusMe.#MSI2021vs C9(@Cloud9) WIN勝ちました!!!!!#DFMWIN pic.twitter.com/rFkd1UyOCi— DetonatioN FocusMe公式 (@teamDFM) May 7, 2021No shame in losing to the best team in Japan. We go again tomorrow.💪 #C9WIN #TrustTheProcess #MSI 📸@lolesports pic.twitter.com/KIXMc6ovHl— Cloud9 (@Cloud9) May 7, 2021C9 is now in desperate need to get back to the true form of their reliable Perkz-Blaber pair. As they are up against Latin American champions Gillette Infinity tomorrow, they will need to bring out their best to stay relevant in the League of Legends MSI 2021.