Fernando Santos' side are currently a point off neighbors Spain with two games to play after beating the Czechs 2-0 in the reverse fixture. They are looking to bounce back following a 1-0 defeat in their most recent clash against Switzerland.
Despite starting just one Premier League game this season, Cristiano Ronaldo is expected to add to his tally of 189 international caps. The forward will also be looking to improve his world record international goal total of 117 but needs to improve on his recent Portugal form.
The five-time Ballon d'Or winner has scored in just one of his national side's previous eight games as they look to prepare for the upcoming FIFA World Cup. Cristiano Ronaldo is one of seven Portugal players who are one booking away from a suspension while Joao Cancelo has already reached the yellow card limit.
How are Portugal and Cristiano Ronaldo preparing for the FIFA World Cup
The Qatar World Cup is almost certainly Ronaldo's last chance to win the one trophy that has eluded him over the course of his extraordinary career.
However, his Portugal teammates will need to improve their dreadful recent record at the tournament, as they haven't won a knockout game since 2006.
Ronaldo has scored just one goal this season in eight appearances, which was a penalty in the Europa League against FC Sheriff. The legendary striker has scored two goals in his previous three appearances against the Czechs and captained his side to victory in the inaugural edition of the Nations League in 2019.
Portugal will be strong favorites to progress from their World Cup group, which consists of Ghana, Uruguay and South Korea.
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