Holger Rune produced a masterful performance to defeat defending champion Novak Djokovic in a thrilling quarter-final match at the 2023 Italian Open on Wednesday, May 17.The 20-year-old Dane won 6-2, 4-6, 6-2 in a match that lasted two hours and 19 minutes. He will next face Casper Ruud or Francisco Cerundolo in what would be his third ATP Masters 1000 semi-final.Djokovic's defeat means that for the first time since 2004, neither he nor the injured Rafael Nadal will compete in the Rome final. The Serb was looking to win his first ATP Masters 1000 title of 2023 in the Italian capital, but it was not to be.Several tennis fans reacted on Twitter to Novak Djokovic's unexpected loss. One user asked if fans should be "worried" about the Serb's chances at the 2023 French Open. "Should we get worried for Novak Djokovic at Roland-Garros?" the user tweeted.We Are Tennis@WeAreTennis Monte-Carlo - R16 Banja Luka - QF Rome - QFShould we get worried for Novak Djokovic at Roland-Garros?424❌ Monte-Carlo - R16❌ Banja Luka - QF❌ Rome - QFShould we get worried for Novak Djokovic at Roland-Garros? https://t.co/2gvxWA6B1PDespite being a fan of Djokovic, another user congratulated Holger Rune and expressed delight that the 22-time Slam champion was able to win at least one set."I'm a nole fan, but i still expected this. congrats nonetheless. I'm glad he was at least able to get a set," the user wrote.lady lazarus@punished_ballet@TennisTV @holgerrune2003 i'm a nole fan, but i still expected this. congrats nonetheless. i'm glad he was at least able to get a set 1@TennisTV @holgerrune2003 i'm a nole fan, but i still expected this. congrats nonetheless. i'm glad he was at least able to get a set 😅According to another account, the Belgrade native has lost his "aura of invincibility" and should put down his racquet."Time for Novak to retire. He’s lost his aura of invincibility. As all great players do in time," the account posted.Lee Williams@leewilliams17@TennisTV @holgerrune2003 Time for Novak to retire. He’s lost his aura of invincibility. As all great players do in time.1@TennisTV @holgerrune2003 Time for Novak to retire. He’s lost his aura of invincibility. As all great players do in time.Here are a few more reactions from tennis fans:Lachhman Bhatia@LachhmanBhatia@TennisTV @holgerrune2003 Another upset in Italian Open. Imagine Djokovic losing third set 2-6 & the match. And yet he's won the tournament record six times. Rune played more aggressively than Djokovic especially in deciding third set. Maybe something is physically wrong with Djokovic. Big setback for FO1@TennisTV @holgerrune2003 Another upset in Italian Open. Imagine Djokovic losing third set 2-6 & the match. And yet he's won the tournament record six times. Rune played more aggressively than Djokovic especially in deciding third set. Maybe something is physically wrong with Djokovic. Big setback for FOKC@Mr_RamakanttThe torch has been passed. The era of Big 3 dominance is officially over. We've been honored to witness the greatest era of all time with our own eyes.Hopefully a lot of great rivalries between the next (current*) gen to come. twitter.com/TennisTV/statu…Tennis TV@TennisTVRUNE-STOPPABLE @holgerrune2003 defeats Novak Djokovic to progress into the #IBI23 semi-finals!5303RUNE-STOPPABLE ⚡️@holgerrune2003 defeats Novak Djokovic to progress into the #IBI23 semi-finals! https://t.co/nEjgM2GtSFThe torch has been passed. The era of Big 3 dominance is officially over. We've been honored to witness the greatest era of all time with our own eyes.Hopefully a lot of great rivalries between the next (current*) gen to come. twitter.com/TennisTV/statu…Lexie_Kat_2022@22_Lex_kate@TennisTV @holgerrune2003 Well played Holger. Sad for Novak, but a deserved win.12@TennisTV @holgerrune2003 Well played Holger. Sad for Novak, but a deserved win.❤️Fez 🧸@mbali_Djokofan@TennisTV @holgerrune2003 Congrats Rune , surprised Nole even got a set with his form I thought he was going to lose in straight sets hope he will be for Roland Garros I think we need Uli back6@TennisTV @holgerrune2003 Congrats Rune , surprised Nole even got a set with his form I thought he was going to lose in straight sets hope he will be 💯 for Roland Garros 🙏 I think we need Uli back2CentsWorth@98CentsChange@TennisTV @holgerrune2003 I love when a player comes on court unbothered by the legend of Djokovic.10@TennisTV @holgerrune2003 I love when a player comes on court unbothered by the legend of Djokovic.Kenneth Orufuo Ezaga@Orufuo@TennisTV @holgerrune2003 Holger becoming the new Nole to Alcaraz's new Nadal.2@TennisTV @holgerrune2003 Holger becoming the new Nole to Alcaraz's new Nadal.rafaelming120062@rafaelming12001@TennisTV @holgerrune2003 Great job ! If young Dane can learn how to control his emotions and how to respect audiences and opponents, he will be very popular tennis superstar, he got great talent, he beat Djokovic twice and prove he is the best !3@TennisTV @holgerrune2003 Great job 👏! If young Dane can learn how to control his emotions and how to respect audiences and opponents, he will be very popular tennis superstar, he got great talent, he beat Djokovic twice and prove he is the best !"I'm looking forward to working on certain aspects of my game" - Novak Djokovic ahead of French Open 2023Novak Djokovic pictured at the Internazionali BNL D'Italia 2023 - Day Ten.After losing to Holger Rune, Novak Djokovic spoke at a press conference about his chances at the French Open. The Serb stated that he was looking to work on certain aspects of his game before the Grand Slam tournament in France."I don't need anything else. Just training and preparing for the biggest clay-court tournament of the season for me. I know I can always play better," he said."Definitely, I'm really looking forward to working on certain aspects of my game, my body and I hope to get to 100%. That's the goal. I always like my odds in Grand Slams against anyone on any surface, best of five. We'll see how it goes," he added.