Rio Olympics 2016: Louis Oosthuizen becomes the latest golfer to drop out
Former World number one Vijay Singh had earlier withdrawn from the event.
Former British Open champion Louis Oosthuizen on Thursday became the third high-profile golfer to withdraw from this summer's Olympic Games in Rio de Janeiro, citing family and schedule issues.
South African Oosthuizen follows what could become a pattern, started last week by Fiji's former world number one Vijay Singh and continued on Tuesday by Australia's 2013 Masters champion Adam Scott.
"I have always represented South Africa with pride so didn't make my decision without a great deal of thought," 2010 British Open winner Oosthuizen said in a statement released by his management company.
"I would like to wish our golfers and all other athletes competing in Brazil all the very best for success."
According to his management company, world number 12 Oosthuizen informed South Africa's Olympic committee of his decision "after long deliberations citing family and schedule issues".
Singh, a three-times major winner, announced last week that the Zika virus was his main reason for skipping the Rio Games.
"I would like to play the Olympics, but the Zika virus, you know..." Singh, 53, told Golf Channel. "I feel bad, I wanted to play and finally decided against it. It's in the middle of the Tour over here and I'm trying to figure out my game."
U.S. health officials have concluded that Zika infections in pregnant women can cause microcephaly, a birth defect marked by small head size that can lead to severe developmental problems in babies.
The World Health Organization has said there is a strong scientific consensus that Zika can also cause Guillain-Barre, a rare neurological syndrome that causes temporary paralysis in adults.
The connection between Zika and microcephaly first came to light last autumn in Brazil, which has now confirmed more than 1,100 cases of microcephaly that it considers to be related to Zika infections in the mothers.
For Scott, a hectic playing schedule from mid-June until the end of August was his biggest cause for concern.
"It's nothing I've ever aspired to do and I don't think I ever will," world number seven Scott told Reuters last year. "It's all about the four majors and I think that's the way it should stay for golf."
The Rio Olympics takes place from Aug. 5 to Aug. 21.