Offensive Positions
| POSITION | STARTER | SECOND | THIRD+ |
| Quarterback | Derek Carr | Jarrett Stidham | — |
| Running Back | Josh Jacobs | Ameer Abdullah | Zamir White Brandon Bolden Brittain Brown Sincere McCormick |
| Fullback | Jakob Johnson | — | — |
| Wide Receiver | Davante Adams | Hunter Renfrow | Mack Hollins DJ Turner Keelan Cole |
| Tight End | Darren Waller | Foster Moreau | Jesper Horsted |
| Left Tackle | Kolton Miller | Jackson Barton | — |
| Left Guard | Alex Bars | John Simpson | — |
| Center | Andre James | — | — |
| Right Guard | Dylan Parham | Lester Cotton | — |
| Right Tackle | Jermaine Eluemunor | Thayer Munford | — |
Defensive Positions
| POSITION | STARTER | SECOND | THIRD+ |
| Left Defensive End | Maxx Crosby | Tashawn Bower | Jordan Jenkins |
| Right Defensive End | Chandler Jones | Clelin Ferrell | Malcolm Koonce Jordan Jenkins |
| Left Defensive Tackle | Andrew Billings | Neil Farrell Jr. | — |
| Right Defensive Tackle | Bilal Nichols | Kendal Vickers | Matthew Butler |
| Strongside Linebacker | Jayon Brown | Luke Masterson | — |
| Middle Linebacker | Denzel Perryman | Blake Martinez | Darien Butler Micah Kiser |
| Weakside Linebacker | Divine Deablo | — | — |
| Right Cornerback | Amik Robertson | Nate Hobbs | — |
| Left Cornerback | Rock Ya-Sin | Anthony Averett | Sam Webb |
| Strong Safety | Johnathan Abram | Roderic Teamer | — |
| Free Safety | Trevon Moehrig | Duron Harmon | Matthias Farley |
Special Teams Positions
| POSITION | STARTER | SECOND | THIRD+ |
| Punter | A.J. Cole | — | — |
| Kicker | Daniel Carlson | — | — |
| Long Snapper | Trent Sieg | — | — |
| Holder | A.J. Cole | — | — |
| Punt Returner | Hunter Renfrow | DJ Turner | — |
| Kick Returner | Ameer Abdullah | — | — |
