Offensive Positions
| POSITION | STARTER | SECOND | THIRD+ |
| Quarterback | Josh Allen | Case Keenum | — |
| Running Back | Devin Singletary | Zack Moss | James Cook Taiwan Jones |
| Fullback | Reggie Gilliam | — | — |
| Wide Receiver | Stefon Diggs | Gabe Davis | Isaiah McKenzie Jamison Crowder Jake Kumerow Khalil Shakir Marquez Stevenson |
| Tight End | Dawson Knox | Quintin Morris | Tommy Sweeney |
| Left Tackle | Dion Dawkins | Tommy Doyle | — |
| Left Guard | Rodger Saffold | Greg Van Roten | — |
| Center | Mitch Morse | — | — |
| Right Guard | Ryan Bates | Bobby Hart | — |
| Right Tackle | David Quessenberry | Spencer Brown | — |
Defensive Positions
| POSITION | STARTER | SECOND | THIRD+ |
| Left Defensive End | Greg Rousseau | Boogie Basham Jr. | — |
| Right Defensive End | Von Miller | A.J. Epenesa | Shaq Lawson |
| Left Defensive Tackle | Ed Oliver | Jordan Phillips | Brandin Bryant |
| Right Defensive Tackle | DaQuan Jones | Tim Settle | — |
| Strongside Linebacker | Matt Milano | Baylon Spector | Andre Smith |
| Middle Linebacker | Tremaine Edmunds | Tyrel Dodson | Tyler Matakevich |
| Weakside Linebacker | Terrel Bernard | Tyrel Dodson | — |
| Right Cornerback | Dane Jackson | Kaiir Elam | Siran Neal |
| Left Cornerback | Taron Johnson | Christian Benford | Cam Lewis Tre'Davious White |
| Strong Safety | Jordan Poyer | — | — |
| Free Safety | Jaquan Johnson | Damar Hamlin | Micah Hyde |
Special Teams Positions
| POSITION | STARTER | SECOND | THIRD+ |
| Punter | Sam Martin | — | — |
| Kicker | Tyler Bass | — | — |
| Long Snapper | Reid Ferguson | — | — |
| Holder | Sam Martin | — | — |
| Punt Returner | Jamison Crowder | Khalil Shakir | — |
| Kick Returner | Isaiah McKenzie | James Cook | — |
